Sixteen teams will discover their opponents in the Champions League knockout draw on Friday at 11:00 GMT.
Arsenal, Liverpool, Chelsea, Manchester City and Tottenham Hotspur are already in the hat, with Newcastle on course to reach the last 16 after a 6-1 win over Qarabag in their play-off first leg.
The eight winners of the two-legged knockout phase play-offs join the top eight sides from the league-phase table in the draw.
Friday's draw will also allocate a side of the bracket for all teams, meaning they will know who they could potentially meet in the quarter-final, semi-final and final.
Last-16 first legs will take place on either 10 or 11 March, with the reverse fixture scheduled for 17-18 March.
The Puskas Arena in Budapest, Hungary will host the 2025-26 final on 30 May.
How does the last-16 draw work?
The eight winners of the play-offs will meet the top-eight finishers from the league phase.
From this point onward the competition adopts a knockout format, with each fixture other than the final contested over two legs.
As with the play-offs, teams' final ranking in the league phase will influence seeding in the last 16, with seeded sides - those that finished in the top eight of the league - being given the advantage of playing second legs at home.
This is the first season that the position in which teams have placed in the league phase will also influence seeding for the quarter-final and semi-finals.
Teams finishing first to fourth at the end of the league phase will be seeded for the quarter-finals, and therefore given the home advantage in their quarter-final tie second legs.
The two sides who finish top and runner-up in the league will also be seeded for the semi-finals, earning them the benefit of playing the second leg of their semis at home should they reach that stage.
If a seeded team does not progress to the quarter or semi-finals, the team that knocks them out will inherit their seeding position.
Who could the Premier League teams face?
Clubs already know who the opponents they could face in the Champions League last 16.
Top seeds Arsenal will be drawn against one of Olympiakos, Borussia Dortmund, Bayer Leverkusen or Atalanta.
Liverpool and Tottenham, who finished third and fourth respectively in the league phase, will meet either Atletico Madrid, Club Brugge, Galatasaray or Juventus.
Manchester City will be drawn against one of Bodo/Glimt, Inter Milan, Real Madrid or Benfica.
Chelsea will take on either Qarabag, Newcastle, Monaco or Paris St-Germain.
If Newcastle do progress through the play-offs, they will face either Chelsea or Barcelona.
Can teams from same country face each other?
Yes, teams from the same nation can face each other in the knockout phase.
Clubs can also face sides who they have already met in the league phase.
Can January signings play?
Players signed in the January transfer window are eligible to feature in the Champions League knockout phase.
It means Manchester City will have both Marc Guehi and Antoine Semenyo available and Tottenham can call upon new addition Conor Gallagher.
Mamadou Sarr is also eligible to feature for Chelsea following his loan recall from Strasbourg.
Clubs had to register the players in their Champions League 'A list' before the knockout play-offs in order for them to participate.
Knockout phase draw and fixture dates
The dates for the knockout phase are as follows:
Last 16: 10-11 and 17-18 March
Quarter-finals: 7-8 and 14-15 April
Semi-finals: 28-29 April and 5-6 May
Final: 30 May
This article is the latest from BBC Sport's Ask Me Anything team.
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